Learning Goal: I’m working on a biology writing question and need guidance to help me learn.
Hello, i need assistance in completing a 3 part writing section. it doesn’t need to be a long paragraph, but something short and concise to the question. Thank you.
Complete the following worksheet and insert your graphed data for full credit. Turn the completed worksheet into the appropriate dropbox by the assigned due date.
Count the number of colonies on each plate. For plates with a large number of colonies, a method to estimate the number will be demonstrated. Post your data on the board in class. You will also need to record the data for the entire class.
What extracts did you test? |
1. Test 1 2. Test 3 |
What was the purpose of the “no treatment” and “minus pGLO” reactions? |
The purpose of the nontreatment and the minus pGL0 was it to be a control in comparison with the other groups we tested. |
Record your Data in the table below – expose your plates to UV light to fully phenotype them
You may estimate the number of colonies if they are very dense.
# of colonies | # of glowing colonies | # ofnon glowing colonies | |
No treatment | 1364 | 1364 | 0 |
minus pGLO (no DNA) | 0 | 0 | 0 |
Extract 1____ | 804 | 804 | 0 |
Extract3_____ | 1132 | 1132 | 0 |
Record the number of glowing colonies from each group
Group 1 (AB,BP,MM,JL) | Group 2 (LH,JH,DT,LK) | Group 3(MC,KD,EC,RR) | Group 4(ENJC) | Group 5(JZJ) | Group6(LO,JT,KW,SM) | |
Notreatment | 1644 | 1364 | 396 | 1620 | 504 | 2,120 |
No pGLO | 0 | 0 | 9 | 0 | 0 | 0 |
Extract 1 | 804 | 1672 | 2,104 | |||
Extract 2 | 2 | 48 | 5 | |||
Extract 3 | 1132 | 2019 | 304 | |||
Extract 4 | 1880 | 2568 | 684 |
Calculate the average number of glowing colonies for each condition and the standard deviation
Average | Stdev | |
No treatment | 1,274.7 | |
No pGLO | 1.5 | |
Extract 1 | ||
Extract 2 | ||
Extract 3 | ||
Extract 4 |
Insert an appropriate graph for your group’s data.
Insert an appropriate graph for the class data where you plot the average and use standard deviation values as error bars.
What can you conclude about the extracts? |
The extracts were |
How might you confirm that the extract that had the greatest affect on the transformation process is actually affecting the DNA and not some other part of the transformation process? Propose a test. |
The test I propose is in a |
Record observations of your plates and point out anything interesting or unexpected you observed. |
In our group plates |
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