Assistance for bio worksheet question

Learning Goal: I’m working on a biology writing question and need guidance to help me learn.

Hello, i need assistance in completing a 3 part writing section. it doesn’t need to be a long paragraph, but something short and concise to the question. Thank you.

Complete the following worksheet and insert your graphed data for full credit.  Turn the completed worksheet into the appropriate dropbox by the assigned due date.

Count the number of colonies on each plate.  For plates with a large number of colonies, a method to estimate the number will be demonstrated.  Post your data on the board in class.  You will also need to record the data for the entire class.

What extracts did you test?
1. Test 1 2. Test 3  
What was the purpose of the “no treatment” and “minus pGLO”
 reactions? 
  The purpose of the nontreatment and the minus pGL0 was it to be a control in comparison with the other groups we tested.      

Record your Data in the table below – expose your plates to UV light to fully phenotype them

You may estimate the number of colonies if they are very dense. 

 # of colonies# of glowing colonies# ofnon glowing colonies
No treatment136413640
minus pGLO (no DNA)000
Extract 1____8048040
Extract3_____113211320

Record the number of glowing colonies from each group

 Group 1 (AB,BP,MM,JL)Group 2  (LH,JH,DT,LK)Group 3(MC,KD,EC,RR)Group 4(ENJC)Group 5(JZJ)Group6(LO,JT,KW,SM)
Notreatment1644136439616205042,120
No pGLO009000
Extract 1 804 1672 2,104
Extract 22 48  5
Extract 3 11322019 304 
Extract 41880  2568684 

Calculate the average number of glowing colonies for each condition and the standard deviation

 AverageStdev
No treatment1,274.7 
No pGLO1.5 
Extract 1  
Extract 2  
Extract 3  
Extract 4  

Insert an appropriate graph for your group’s data.

Insert an appropriate graph for the class data where you plot the average and use standard deviation values as error bars.

What can you conclude about the extracts?   
The extracts were      
How might you confirm that the extract that had the greatest affect on the transformation process is actually affecting the DNA and not some other part of the transformation process?  Propose a test.
The test I propose is in a        
Record observations of your plates and point out anything interesting or unexpected you observed.   
In our group plates        

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