I’m working on a biology question and need an explanation to help me study.

A new graduate student is asked to add a nuclear localization sequence (NLS) to the C-terminus of a secreted protein in order to localize the protein to the nucleus. What would be the most appropriate response?

Group of answer choices

This will not work since the NLS will be cleaved off the protein.

Great idea, but we should remove the endoplasmic reticulum signal sequence.

Great idea, we can now see if this protein can function in the nucleus.

Great idea, but the NLS will act as a transmembrane domain.

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